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KMID : 0545119960060040270
Journal of Microbiology and Biotechnology
1996 Volume.6 No. 4 p.270 ~ p.277
Isolation of Leuconostoc mesenteroides subsp. mesenteroides DU-0608 with Antibacterial Activity from Kimchi and Characterization of Its Bacteriocin
Cha Dong-Soo

HA DUK-MO
Abstract
A bacteriocin-producing strain, DU-0608, was isolated from Kimchi and identified as Lauconostoc mesenteroides subsp. mesenteroides. The bacteriocin from isolate was inhibitory against Listeria monocytogenes, Micrococcus luteus and several strains of lactic acid bacteria. The bacteriocin was inactivated by pepsin, trysin, ¥á-chymotrypsin, protease, ¥á-amylase and lipase, but not by catalase or by heating at 100¡É for 60 min. The molecular weight of the bacteriocin was estimated approximately 6 kDa. The inhibitory effect was bactericidal and rapid. Following treatment with isolate bacteriocin, cells of indicator strain (Lactobacillus sake JCM 1157) were damaged at the end regions of the cell wall, whereas the cells treated with nisin were damaged at many places around the cell wall.
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